PrimeMate: Fast and Streamlined Primer Generator for Ensembl
PrimeMate is a Chrome add-on developed by PrimeMate that serves as a primer generator for Ensembl. It allows users to generate hundreds of primer pairs with customizable parameters and sort them according to their preferences.
With PrimeMate, users can easily work with the Ensembl genome database by simply navigating to the desired sequence and clicking "find primers". The add-on offers a range of customizable options such as optimal product length, melting temperature, G/C content, and dimerization/hairpin threshold.
One of the standout features of PrimeMate is its ability to display detailed information for each product, including length, melting temperature, and exon location. Users can also sort the generated results based on their preferred criteria by adjusting the sorting weight for each variable.
PrimeMate goes the extra mile by automatically filtering undesirable products, such as self-dimerizing primers or those with suboptimal locations on the sequence. It also provides the option to save user-selected sequences in a "Favorites" row for easy access and allows for seamless sharing by copying the sequences to the clipboard.
Overall, PrimeMate is a powerful and efficient tool for primer generation in Ensembl, offering a user-friendly interface and a wide range of customizable options.
